Promoter |
T7, SP6 |
Includes |
Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot TOP10F' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. |
Content And Storage |
This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Format |
Kit |
Product Type |
Cloning Kit |
Cloning Method |
TA Cloning |
Bacterial or Yeast Strain |
TOP10F ́ |
For Use With (Application) |
PCR Cloning |
Vector |
pCRII |
Product Line |
One Shot™ |
Cell Type |
Chemically Competent E. coli |